Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
권호 표지

Partial Purification and Characterization of a Glycoprotein Factor from Fresh Ginseng

수삼으로부터 당단백질 인자의 부분정제와 특성연구

  • Kong, Yun-Cheung (Department of Biochemistry The Chinese University of Hong Kong) ;
  • Fong, Wing-Ping (Department of Biochemistry The Chinese University of Hong Kong) ;
  • Song, Myung-Eun (Department of Biochemistry The Chinese University of Hong Kong) ;
  • Ng, Kam-Hung (Department of Biochemistry The Chinese University of Hong Kong) ;
  • Ho, Dan-Dan (Department of Biochemistry The Chinese University of Hong Kong) ;
  • Ng, Ping-Chung (Department of Biochemistry The Chinese University of Hong Kong)
  • Published : 1990.08.01
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Abstract

The aqueous extract of fresh ginseng (Panax ginseng C.A. Meyer) contains a macromolecular fraction that showed mitogenic and co-mitogenic activities in human peripheral blood lymphocytes. Purification of the crude extract by size (ultrafiltration, Sephadex G-200) and charge (DEAE-cellulose, DEAE-Sepharose) yielded a semi.purified fraction (DS-3). This fraction contains at least three subgroups of anionic macromolecules with apparent molecular weight greater than 600 kilodaltons. It is a glycoprotein with a large amount of glucuronic acid. It acts as a mitogen in both T and B cells of human peripheral blood lymphocytes. It could also potentiate the mitogenic action of Concanavalin A in lymphocyte T cells. Such potentiation is not due to increased binding of Concanavalin A to the cell surface. Its mitogenic and co-mitogenic effects do depend on the presence of extracellular Ca2+.

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