Bacillus속 AP-5 균주의 고온성 알카리 배양에 따른 Alkaline Pretense의 생성 및 성질

Production and Properties of Alkaline Pretense from Bacillus sp. Strain in Thermophilic and Alkaline Condition

For production of thermophilic and alkaline protease, Bacillus sp. strain AP-5 was isolated from a compost. The production of the protease was reached at maximum for 4 days at $55^{\circ}$ in standing culture. Chitin and Cellulose as carbon source, and Skim Milk as nitrogen source were favorable for the production of the enzyme. Optimal temperature and optimal pH of the enzyme was $55^{\circ}$ and 11, respectively. Metal ion didn't effect on the enzyme activity, the protease was very stable at heat treatment of 30 min at $55^{\circ}$.