미생물학회지 (Korean Journal of Microbiology)
- 제28권2호
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- Pages.98-103
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- 1990
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Brevibacterium lactofermentum의 원형질체 융합에 의한 유전자 재조합
Genetic Recombination of Brevibacterium lactofermentum by Protoplast Fusion
초록
원형질체 융합에 사용할 genetic selection marker로써 자외선 조사와 NTG 처리에 의해 Brevibacteriurn lactofermeηturn S SWA(arg trp)와 B. lactofermenturn SWB(met ser)를 분리하였다. 이 균주릎 배양하여 증식기에 penicillin G를 처리한 후, 0.4 M sucrose를 첨가한 lysis fluid에서 lysozyme을
Brevibacterium lactofermentum SWA (arg trp) and B. lactofermentum SWB (met ser) were obtained from UV and NTG treatment. The rates of protoplast formation by B. lactofermentum SWA and SWB were 99.93% and 99.98%, respectively when each strain was treated with penicillin G in mid exponential growth phase, followed by incubation with 400