Conversion of Plant Sterols to Androsta-4-ene-3,17-dione by a mutant of Mycobacterium sp. NRRL B-3805

Mycobacterium종 (NRRL B-3805)의 변이종에 의한 식물스테롤의 androsta-4-ene-3,17-dione(AD)으로의 전환

A mutant was selected by NTG treatment of Mycobacterium sp. NRRL B-3805, which was capable of degrading plant sterol to androsta-4-ene-3, 17-dione and yields was higher than NRRL B-3805. Also this mutant produced androst-4-ene-3, 17-dione faster than NRRL B-3805. It described the mode of sitosteroidal degradation, and the interrelation between cell membrane and its attachment to substrate during the sterol degradation process by this mutant and it was compared with Mvcobacterium sp. NRRL B-3805.