KSBB Journal

  • 제5권3호
  • /
  • Pages.255-261
  • /
  • 1990
  • /
  • 1225-7117(pISSN)
  • /
  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Phaffia rhodozyma의 원형질체 융합

Protoplast Fusion of phaffia rhodozyma

  • 배석 (전남대학교 미생물학과) ;
  • 김문휘 (전남대학교 생물학과) ;
  • 박종천 (전남대학교 생물학과) ;
  • 김재형 (전남대학교 공업화학과) ;
  • 전순배 (전남대학교 미생물학과)
  • Bai, Suk (Dept. of Microbiology, Chonnam National University) ;
  • Kim, Moon-Whee (Dept. of Biology, Chonnam National University) ;
  • Park, Jong-Chun (Dept. of Biology, Chonnam National University) ;
  • Kim, Jae-Hyung (Dept. of Chemical Technology, Chonnam National University) ;
  • Chun, Soon-Bai (Dept. of Microbiology, Chonnam National University)
  • 발행 : 1990.11.01
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초록

Astaxanthin을 생산하는 효모 Phaffia rhodozyma로부터 제조된 상보적 돌연변이 균주간의 세포융합을 통하여 astaxanthin을 대량 생산하는 균주를 얻고자 시도하였다. 이들의 원형질체 융합빈도는 $1.3$\times$10^-^5-6.0$\times$10^-^5$이었고 DNA함량, 핵염색, UV조사에 대한 생존력 비교 그리고 형질분리분석등으로 핵융합을 확인하였다. 융합체 중 F1은 야생형의 모 균주와 비교했을때 astaxanthin생성량이 약 3배 증가하였다.

Cell fusion between complementary mutants isolated from astaxanthin-producing yeast, Phaffia rhodozyma, was carried out to obtain astaxanthin-overproducing strains by protoplast fusion technique. The frequency of protoplast fusion was ranged from 2.3$\times$10-5 to 6.0$\times$10-5, and nuclear fusion in the cells of hybrids was demonstrated by several techniques such as isolation of recombinants after mitotic segregation of parental genetic markers, estimation of DNA content, direct observation of nuclei with nuclear staining, and comparison of survival rate to UV exposure. One of several hybrids, Fl, showed approximately 3-fold increase in astaxanthin content when compared with wild parent.

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