한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제18권6호
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- Pages.653-659
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- 1990
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Schwanniomyces castellii 전분 유전자의 Cloning과 발현
Cloning and Expression of Schwanniomyces castellii Starch Gene
- Park, Jong-Chun (Department of Microbiology, Chonnam National University) ;
- Bai, Suk (Department of Microbiology, Chonnam National University) ;
- Chun, Soon-Bai (Department of Microbiology, Chonnam National University)
- 발행 : 1990.12.01
초록
Schwanniomyces castelli CBS 2863의 glucoamylase 유전자를 Saccharomyuces cerevisiae에 cloning하고 발현시켰다. Southern blot 분석결과, 형질전환체의 glucoamylase 유전자는 Sch. castellii genomic DNA로부터 나온 것임을 확인하였고 5.1 혹은 1.3kb의 Sch.castellii 유전자에 해당되는 DNA 절편이 S.cerevisiae 에서 관찰되지는 않았다. S.cerevisiae의 형질전환체의 glucoamylase 활성은 Sch. castellii의 그것에 비해 2,000배 정도 낮았고 E.coli에서는 발현되지 않았다. Sch.castellii의 glucoamylase와 동일한 특성과 분자량을 가지고 있음을 알 수 있었다.
The gene encoding glucoamylase from Schwanniomyces cagtellii CBS 2863 was cloned and expressed in Saccharomyces cerevisiae. Southern blot analysis confirmed that this glucoamylase gene was derived from the genomic DNA of Schwanniomyces ccastellii and that no DNA fragments corresponding to 5.1 or 1.3 kb of Sch. casteltii DNA were detected in S. cereuisiae. The glucoamylase activity from S. cerevisiae transformant was approximately 2,000 times less than that of donor yeast. No expression was found in E. coti. The secreted glucoamylase from S. cerevisiae transformant was indistinguishable from that of Sch. eastellii on the basis of molecular weight and enzyme properties.