Preventive Effect of Lysis in the Cadmium-Tolerant Hansenula anomala B-7 Cells by Metal Ions

금속 이온에 의한 카드늄 내성균주 Hansenula anomala B-7 세포의 용균 방지 효과

The yeast, Hansenula anomala B-7, isolated from the $Cd^{2+}$ rich soils and determined to be tolerant in the high concentration of $Cd^{2+}$ were employed in this work. Its intact cells grown in high concentration of $Cd^{2+}$ were observed to be Iysed at the early stage when transferred to a cadmium deficient broth. Its intact cells found to be not Iysed and grow well under the high concentration of $Cd^{2+}$. The Iysis of the intact cells grown at the high concentration of $Cd^{2+}$ ion was not found when the metal ions were replaced with $Cd^{2+}$ ion in the same concentration. This result indicated that Iysis of yeast cells, at least in this isolate, would be related to cell osmosis with the mineral ions added.

$1,000\;{\mu}g/ml$의 카드늄이 함유된 배지에서 배양된 고도 카드늄 내성 Hansenula anomala B-7의 intact cell은 카드늄이 함유되지 않은 배지에 재배양하면 쉽게 용균된다. 그러나 이 intact cell을 $1,000\;{\mu}g/ml$의 카드늄이 함유된 배지에 배양하면 용균이 일어나지 않을 뿐 아니라 왕성하게 재증식 한다. 더욱이 재배양할시 $Na^+$를 포함한 다른 금속을 배지에 첨가하더라도, 이 intact cell의 용균은 방지 되었다.