Isolation and Characterization of Biopolymer Producing Alkali-Tolerant Bacterial Strain

생물고분자 생산 알칼리 내성 균주의 분리 및 특성

  • Lee, Shin-Young (Department of Fermentation Technology and Engineering, Kangweon National University) ;
  • Lee, Beom-Su (Department of Fermentation Technology and Engineering, Kangweon National University) ;
  • Shin, Weon-Chul (Department of Fermentation Technology and Engineering, Kangweon National University) ;
  • Kwon, Ik-Boo (Lotte Group R & D Center) ;
  • Yu, Ju-Hyun (Department of Food Engineering, Yonsei University)
  • Published : 1991.04.01

Abstract

For the production of useful products from microorganism, a bacterial strain producing the biopolymer was isolated from soil. The bacteriological characteristics of the strain were examined and some chemical properties of the biopolymer produced were investigated. The bacterial strain was identified as an alkali-tolerant Bacillus sp. The results of chemical composition, various color reactions and I.R. spectrum revealed that the biopolymer contained high protein content, low amino sugar and no uronic acid. However, the biopolymer was precipitated by treating with cetylpiridinium chloride and was found to be acidic.

미생물을 이용한 유용물질 생산연구의 일환으로 토양으로부터 생물고분자 생산균주를 분리하고, 이 분리균에 대한 균학적 성질 및 생성 생물고분자의 이화학적 성상을 검토하였다. 분리균주는 Bacillus속의 한 균종으로 동정 하였으며, 알칼리 내성균주의 특징을 나타내었다. 이 균주로부터 생성된 생물고분자는 성분분석, 정색반응 및 I.R. spectrum 분석결과 단백질 함량이 높고, 일부 amino sugar가 존재하나 uronic acid는 함유하지 않았다. 그러나 cetyl pyridinium chloride에는 침전되어 산성의 생물고분자인 것으로 추정되었다.

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