Productivity of Polysaccharide by Mutant of Acetobacter pasteurianus IFO 13751

Acetobacter pasteurianus IFO 13751의 돌연변이주에 의한 다당류 생산성

In order to obtain the highest productivity of polysaccharide, acetic acid bacteria was used. Several acetic acid bacteria were investigated to the productivity of polysaccharide, an mutant, Acetobacter pasteurianus IFO 13751-5 selected among serveral acetic acid bacteria which can produce the polysaccharide by radiation of ultra-violet ray. Acetobacter pasteurianus IFO 13751-5 was shown 3 fold polysaccharide production than that of its parents. When the Acetobacter pasteurianus IFO 13751-5 was investigated under the condition of carbon source containing 5% sucrose, the highest amount of polysaccharide (45.95 mg/ml) was obtained. The polysaccharide production by Acetobacter pasteurianus IFO 13751-5 was 55.10 mg/ml by using jar fermentor.

초산균의 변이주 중 다당류 생산량이 우수한 균수를 선별한 결과 Acetobacter pasteurianus IFO 13751-5가 친주보다 약 3배의 다당류 생산량을 나타내었다. 돌연변이주인 Acetobacter pasteurianus IFO 13751-5의 최적 배양조건을 검토한 결과 탄소원은 sucrose로써 5% 첨가시 45.95 mg/ml로 가장 높게 나타났다. 다당류를 대량생산할 목적으로 jar fermentor를 이용하여 생산된 다당류는 72시간에서 55.10 mg/ml의 최대 생산량을 나타내었다. 이 배양액을 분리 정제하여 진공 동결건조한 다당류는 18.93 g/l을 얻었다.