Production of $poly-{\beta}-hydroxybutyric$ acid by methanol assimilating bacterium, Pseudomonas sp. ILS-003

Methanol 자화성 세균 Pseudomonas sp. ILS-003에 의한 $poly-{\beta}-hydroxybutyric$ acid의 생산

  • Lee, Il-Seok (Department of Agricultural Chemistry, College of Agriculture, Korea University) ;
  • Bang, Won-Gi (Department of Agricultural Chemistry, College of Agriculture, Korea University)
  • Published : 1991.09.30

Abstract

Methanol assimilating bacterium, Pseudomonas sp. ILS-003 was used to investigate the optimum conditions for the production of $poly-{\beta}-hydroxybutyric$ acid from methanol. For PHB production, the optimum initial pH was 6.4 and the optimum temperature was $30^{\circ}C$. Also the optimum methanol concentration was found to be 1.0%(v/v). In the PHB production, $(NH_4)_2SO_4$ was the most effective nitrogen source and the optimum concentration was 0.8 g/l, which was eqivalent to 17.4 in C/N ratio. Also, deficiency of the 2 valence metal ions in the medium had stimulating effect on PHB accumulation. Under the optimum substrate concentration, successive feeding of 0.25%(v/v) methanol was the most effective on PHB production. Under the optimum conditions, 1.94 g/l of PHB and 2.78 g/l of dry biomass were produced in 96 hours, and the yield was 69.8%(w/w).