Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Patterns of Soluble Protein, Reducing Sugar and Ginsenosides in Transformed Calli of Ginseng (Panax ginseng C.A. Meyer

형질전환 인삼 Callus의 단백질, 환원당 및 Ginsenoside의 양상

  • Yang, Deok-Jun (Dept. of Genetics & Physiology, Korea Ginseng & Tobacco Research Institute, ) ;
  • Choe, Gwang-Tae (Dept. of Genetics & Physiology, Korea Ginseng & Tobacco Research Institute, ) ;
  • Yang, Deok-Deok (Department of Biology, Chungbuk National Unversity)
  • 양덕준 (한국인삼연초연구소 유전생리부) ;
  • 최광태 (한국인삼연초연구소 유전생리부) ;
  • 양덕ㅈ (충북대학교 생물학과)
  • Published : 1991.08.01
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Abstract

This study was conducted to obtain basic information about the transformation of ginseng tissue, identification of opine compound and protein, and saponin production from ginseng callus transformed with Ti-plasmic of AW$.$obacterium tumefaiens C58. Ginseng crown gall callus induced by pTiC58 could be continuously cultured on the Phytohormone-free medium. The transformation was reconfirmed by the detection and identification of opine compound, from the gall callus. The transformed ginseng callus contained higher amounts of protein than normal callus and the protein pattern of transformed callus was quite different from that of normal callus. The xylose which is not detected in the normal callus and ginseng root was identified in gall callus. The saponin contents of gall callus of ginseng were three times higher than that of normal callus, and ginsenoside composition of the transformed callus was similar to that of the cultivated ginseng root, but quite different from that of normal callus.

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