Immunological Studies on the Surface Antigens of Tumor Cell (II) Introduction to Immunological Studies on the Development and Cell Differentiation of the Leukemia Cell

종양세포 표면항원에 대한 분자면역학적 연구(II) 백혈병세포의 발생과 세포분화에 관한 연구

The CALLA on the surface of leukemic cell lines, recognized by our monoclonal antibody. KP-22(IgG1, K) was one of cell surface glycoproteins having moi. wt. of approximately 100,000 dalton, and could be shed in spent medium or endocytosed when binding the cognate antidoby, KP-22. In the presence of cognate antibodies, 60% of CALLAS recogniEed by KP-22 MAs were modulated and cleared from the cell surface during 24 hrs, and approximaetely 35% of them was endocytosed and 25%, was shed in spent medium. The reappearance of the membrane CALLA after modulation by the KP-22 required at least 6 hours and supposed to be newly synthesized molecules.