Korean Journal of Microbiology (미생물학회지)
- Volume 29 Issue 2
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- Pages.117-122
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- 1991
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Improvement of bacteria for removing of phosphate by spheroplast fusion
원형질체 융합에 의한 인산염 제거균주의 개량
Abstract
In order to improve the removal ability of phosphate, Spheroplast fusions were performed among auxotrophic mutants of Aeromonas hydrophila isolated from waste water, named A13 and A14, Aci37 auxotrophic mutant of Acinetobactercalcoaceticus, and auxotrophic E. coli HR262/pCE27 carring pit gene. Eight fusants obtained from this experiment showed different biochemical characteristics. When the rate of phosphate uptake among fusants (F1-F8) was investigated in Phosphate Uptake Medium (PUM), F8 strain showed the highest rate for phosphate removal, 7 times as much as control after two hours incubation. The role of cations (
Keywords
- Aeromnas hydrophila;
- Acinetobacter cllcoaceticus;
- Escherichia coli Hr262/ pCE27;
- phosphate uptake midium(PUM);
- phosphate releasing medium (PRM)pit;
- pst