Induction and Free Amino Acid Analysis of Callus from Canavalia lineata Leaf

해녀콩(Canavalia lineata) 잎 칼루스의 유도와 유리 아미노산의 분석

The callus of Canavalia lineata was induced from leaf tissues in MS medium supplemented with 10-5 M kinetin and 10-6 M IAA and was subcultured in Miller's medium supplemented with 10-5 M BAP and 10-6 M 2,4-D. When free amino acids of callus were analysed by HPLC, canavanine was not detected in the callus cultured either in the dark or light. But exogenously supplied canacanine was accumulated or consumned in the callus of Canavalia lineata.