Characteristics, Mass Transfer Coefficient and Effectiveness Factor of $\beta$-glucosidase Immobilized on the Diazotized Chitin in Bioreactors

Diazotized Chitin에 고정된 $\beta$-glucosidase의 생물반응기에서의 특성, 물질전달계수 및 효율인자에 관한 연구

Diazotized chitin(CHITN) as supports of immobilized enzyme, which was obtained by alkaline hydrolysed chitin with NaN3 and HCI was employed to produce CHITN-Gase with glutaraldehyde as bifunctional reagent. Activities of CHITN-Gase were determined with reaction of p-nitro-pheol-$\beta$-D-glucopyranoside(PNG) in plug flow reactor as a reference of CHITA-Gase. Their optimum temperature, pH, Km and Vmax, mass transfer coefficient (h), effctiveness factor(η)were plotted with variation of flow rate and H/D. Mass transfer coefficient(h) of those enzymes increased because of their flux, as flow rates were increased and controlled by reaction rate. Effectiveness factor(η) of both enzymes were nearly 1.0.