농촌의학ㆍ지역보건 (Journal of agricultural medicine and community health)
- 제16권1호
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- Pages.70-78
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- 1991
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- 1738-9577(pISSN)
Anisakis 감염(感染) 가토(家兎)의 시기별(時期別) 항체검출(抗體檢出)에 대한 각종 항원(抗原)의 적용성(適用性)
Application of Various Antigens on the Detection of Antibody in Rabbits Infected with Anisakid Larvae
- 전복실 (중화인문공화국 길림성 연변의학원 기생충교연실) ;
- 정명숙 (고려대학교 의과대학 기생충학교실 및 열대풍토병연구소) ;
- 주경환 (고려대학교 의과대학 기생충학교실 및 열대풍토병연구소) ;
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임한종
(고려대학교 의과대학 기생충학교실 및 열대풍토병연구소)
- Quan, Fu-Shi (Department of Parasitology, Yan Bian Medical College, Province Jilin, People's Republic of China) ;
- Chung, Myung-Sook (Department of Parasitology and the Institute for Tropical Endemic Diseases, College of Medicine, Korea University) ;
- Joo, Kyoung-Hwan (Department of Parasitology and the Institute for Tropical Endemic Diseases, College of Medicine, Korea University) ;
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Rim, Han-Jong
(Department of Parasitology and the Institute for Tropical Endemic Diseases, College of Medicine, Korea University)
- 발행 : 1991.06.30
초록
Antibody changes in experimental anisakiasis were observed by ELlSA and SDS-PAGE/EITB using various antigens : whole worm extract antigen(WWE), somatic antigen(SOM), excretory-secretory antign(ES), and hemoglobin antigen(HB) of Anisakis Type 1. The results obtained were as follows. l) Serum levels of IgG antibody by ELISA increased from 1st week of infection and reached their maximum titer at 5th week after infection, and decreased gradually thereafter. 2) The best result expressed as positive/negative ratio could be obtained when ES antigen was used. 3) Silver stained SDS-PAGE of each antigen showed at least 20 protein bands : In WWE, 286, 278, 262, 38, 18 Kd bands ; In SOM, 38 Kd band : In ES, 286, 65, 13 Kd bands ; In Hb, 61, 55, 38, 28, 26, 22, 20, 16, 15 Kd bands iepntibied as were major bands. 4) By EITB using WWE, Serum antibody recognized major protein with molecular weight of 86 Kd and 16 Kd. Using ES, 69, 59, 16 Kd bands were observed and using Hb, 28 Kd band was observed as specific band. In conclusion, excretory-secretory antigen(ES) of Anisakis larvae was most usable for ELISA.
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