Localization and Activity of Alkaline Phosphatase and Adenosine Triphosphatase of Ovarian Follicles in Pig

돼지 난포내 Alkaline Phosphatase 및 Adenosine Triphosphatase의 활성부위와 활성도

  • Kim, Moon-Kyoo (Department of Biology, College of Natural Sciences, Hanyang University) ;
  • Gye, Myung-Chan (Department of Biology, College of Natural Sciences, Hanyang University) ;
  • Yoon, Hyun-Soo (Department of Biology, College of Natural Sciences, Hanyang University) ;
  • Kim, Jong-Heup (Department of Biology, College of Natural Sciences, Hanyang University)
  • 김문규 (한양대학교 자연과학대학 생물학과) ;
  • 계명찬 (한양대학교 자연과학대학 생물학과) ;
  • 윤현수 (한양대학교 자연과학대학 생물학과) ;
  • 김종흡 (한양대학교 자연과학대학 생물학과)
  • Published : 1991.12.31

Abstract

In order to study the growth and maturation of ovarian follicle, the localization and activity of alkaline phosphatase(ALPase) and adenosine triphosphatase(ATPase) of the granulosa cells and theca layer were examined according to the follicle size, the follicle state and the ovarian cyclic phase in pig. Theca interna of the small follicles was more heavyly localized with reaction product by the activites of ALPase and ATPase than that of the large follicles. It is assumed that, as the follicles proceed to growth and maturation, antrum formation is the result of the follicular fluid accumulation by means of active transport by the activities of ALPase and ATPase in theca interna. The activities of ALPase and ATPase in atretic follicles were higher than those of normal follicles. These results imply that the mechanisms of follicle maturation and atresia are different according to the phase of ovarian cycle.

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