대장균(JM 109)에서 효모 Thiol-Specific Antioxident 단백질의 발현

Expression of Yeast Antioxidant Protein Gene in E. coil

발현 Vector인 pKK223-3를 이용하여 효모 Thiol-Specific Antioxidant단백질 유전자를 대장균에 도입시켜 이 단백질을 발현시켰다. 이 단백질은 대장균 단백질의 약 1% 정도로 발현되었으며, 물리 및 화학적 특성은 효모의 것과 동일한 특성을 보였다.

The expression of the cloned Saccharomyces cerevisiae antioxidant protein gene in E. coil on plasmid was studied. Introduction of the DNA encoding yeast thiol-specific antioxidant protein into expression vector, PKK 223-3, having the tac promoter resulted in the production in E. coil of a Mr 27,000 polypeptide. This protein represented as much as about 1% of the bacterial soluble protein and showed the thiol-specific antioxidant properties. The bacterial protein showed physico-chemical properties indistringuishable from those of the yeast protein.