Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation of the Zearalenone-Producing Strains from Agricultural Products in Youngnam Districts

영남지방 농산물에서의 Zearalenone 생성균의 분리

  • Kim, Sung-Young (Department of Foods Science and Technology, Gyeongsang National University) ;
  • Chung, Sun-Hee (Department of Foods Science and Technology, Gyeongsang National University) ;
  • Chung, Duck-Hwa (Department of Foods Science and Technology, Gyeongsang National University)
  • Published : 1992.12.01
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Abstract

To isolate zearalenone-producing strains from agricultural products in Youngnam districts, various samples such as rice(60), barley(52), soybean(45), peanut(33), corn(32), maeju(27), unhulled rice(UR, 40), unhulled barley(UB, 54), and soil(32) collected. From 375 samples, 302 Fusarium strains were isolated. The isolated strains were cultured for 14 days at $30^{\circ}C$ in rice medium, and then screened zearalenone-producing strains by TLC and HPLC. The result was that 29 isolates[rice(3), maeju(2), corn(1), barley(5), soil(4), peanut(3), soybean(4), U.B.(5), U.R.(2)] were screened as zearalenone-producing strains by TLC, while 19 isolates [rice(2), maeju(1), corn(1), barley(4), soil(3), soybean(2), U.B.(3), U.R.(3)] by HPLC method.

영남지방으로부터 백미(60), 보리쌀(52), 대두(45), 땅콩(33), 옥수수(32), 메주(27), 벼(40), 보리(54) 및 토양(32) 등 총 375종의 시료를 수거하여 Fusarium속으로 추정된 균주를 분리하고, 분리균을 고체배지에 접종하여 배양(14일, $30^{\circ}C$)한 후에 zearalenone 생성균주를 선별한 결과는 다음과 같다. 375종의 시료에서 302주의 Fusarium속을 분리하였고, 분리된 302주를 고체배지에서 배양한 다음 TLC와 HPLC법에 의해 zearalenone 생성균주를 선정하였다. TLC 결과에서 zearalenone 생성균주는 29주 [쌀(3), 메주(2), 옥수수(1), 보리쌀(5), 토양(4), 땅콩(3), 대두(4), 보리(5) 그리고 벼(2)]로 나타났으나, HPLC 분석결과 19주 [쌀(2), 메주(1), 옥수수(1), 보리쌀(4), 토양(3), 대두(2), 보리(3) 그리고 벼(3)]가 zearalenone 생성균주로 확인되었다.

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