Archives of Pharmacal Research
- Volume 15 Issue 3
- /
- Pages.229-233
- /
- 1992
- /
- 0253-6269(pISSN)
- /
- 1976-3786(eISSN)
Determination of the pKa for Histidine-51 Residue in the Ternary Compelx of Horse Liver Alcohol Dehydrogenase
- Lee, Kang-Man (College of Pharmacy, Ewha Womans University) ;
- Son, Su-Yeon (College of Pharmacy, Ewha Womans University)
- Published : 1992.09.01
Abstract
The pKa value of histidine-51 residue was determined by the pH dependency of contents of NADH bound to the active site in the orse liver alcohol dehydrogenase and % inactivation with diethyl pyrocarbonate treatment of the enzyme. The pKa for His-51 was -7.15 in the ternary complex and -6.7 in the enzyme itself.