한국동물학회지 (The Korean Journal of Zoology)

한국통합생물학회 (The Korean Society for Integrative Biology)
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인터페론 알파에 대한 단세포 군항체의 제조 및 특성

Production and Characteriuation of Monoclonal Antibodies against Human Interferon-$\alpha$

  • Park, Kyung-Hee (Department of Biology, College of Natural Sciences, Chung-Ang University, Seoul 156-756) ;
  • Lee, Ihn-Sook (Department of Biology, College of Natural Sciences, Chung-Ang University, Seoul 156-756)
  • 발행 : 1992.01.01
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초록

Seven monoclonal antibodies were produced by fusing splenocytes from Balb/C mouse immunized with partially purified human interferon-a (HUIFN-a) with NSO plasmacytoma cells. aery were identified as five IgG class (432.22: IgG2b/n, 460.52: IgG2b/a , 548.46: IgG2a/n , 573.10: IgG2b/h , 625.12: IgG2b/n ), one IgA class (460.50: IgA/n ) and one IsM class (465.27: IgA/n ), and all of them revealed highly sensitive to HUIFN- a IgG class monoclonal antibodies have pts ranged from 8.2 to 8.6. Ascites fluids produced from primed Balb/c mice and were purified through column chromatography. The cytopathic effect (CPE) inhibition assay to examine neutralization of HuIFU-a by IgG class monoclonal antibodies, gave that MAbs 460.52, 548.46, 573.10 can neutralize HUIFU- a arith varying degrees except 432.22. Therefore, it is deduced that these various monoclonal antibodies may recognize the distinct epitopes on HUIFN-a.

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