한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제20권5호
  • /
  • Pages.543-550
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  • 1992
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Asperillus niger NRC 107에서의 Xylanase와 $\beta$-Xylosidase의 생산

The Production of Xylanase and $\beta$-Xylosidase by Aspergillus niger NRC 107

  • 발행 : 1992.10.01
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초록

Hemicellulose를 효소에 의하여 분해기키기 위하여 Aspergillus niger NRC 107로부터 xylanase와 $\beta$-xylosidase의 생산조건에 대하여 조사하였다. 이들 효소 생산 최적 pH는 6.0이었으며 탄소원 중 corn-cob xylan과 질소원 중 $NaNO_{3}$이 효소 생산에 제일 좋은 기질이었으며 이들의 최적농도는 각각 15g/l와 2.67g/l이다. 이들 효소는 인산염($KH_2P0_4$)과 Tween-80의 농도를 조절하므로 수율을 높일 수 있으며 wheat bran은 xylanase의 생산에L(-) sorbose는 $\beta$-xylosidase의 생산에 좋은 영향을 주었다. 이와 같이 이 Aspergillus niger NRC 107의 회분식 배양에서 이들 효소에 영향을 미치는 생산조건을 최적화한 결과 xylanase는 39.43 units/ml, $\beta$-xylosidase는 4.2units/ml 까지 생산할 있었다.

The production of xylanase and $\beta$-xylosidase was investigated in submerged culture of Aspergillus niger NRC 107. The maximum production occurred when the pH was controlled at 6.0 during the fermentation. Among the various carbon sources investigated, corn-cob xylan (1.5%, w/v) yielded maximal production of the enzymes. The $NaNO_{3}$ was the most favorable nitrogen source for enzyme production and $KH_2P0_4$ concentration at 0.3%(w/v) was found to be optimum. Incorporation of wheat bran to the culture medium improved xylanase production. Addition of L( -) sorbose to the culture medium promoted the secretion of $\beta$-xylosidase. It was possible to increase the production of xylanase (39.43 units/ml) and that of $\beta$-xylosidase (4.2 unitslml) by submerged culturing the A. niger NRC 107 in the modified medium.

키워드

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