Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning of Antagonistic Genes in Pseudomonas maItophiliQ B-14

토양병해 길항성 Pseudomonas maltophilia B-14의 길항유전자탐색

  • 구본성 (농업유전공학연구소 분자유전과) ;
  • 서영우 (농업유전공학연구소 분자유전과) ;
  • 윤상홍 (농업유전공학연구소 분자유전과) ;
  • 박경수 (조선대학교 자연과학대학 생물학과) ;
  • 은무영 (농업유전공학연구소 분자유전과) ;
  • 김용환 (농업유전공학연구소 분자유전과) ;
  • 오상우 (농업유전공학연구소 분자유전과) ;
  • 류진창 (농업유전공학연구소 분자유전과) ;
  • 은무영 (농업유전공학연구소 분자유전과)
  • Published : 1992.12.01
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Abstract

Tn5 lac 삽입으로 채소입고병원균에 길항력이 약화된 T-67 및 고추역병균과 참깨역병균에 길항력이 약화된 T-81의 Tn5 lac 유전자 일부와 오른쪽 말단에 있는 길항관련 유전자의 flanking sequence가 cloning된 pAG67 및 pAG81 clone을 선발하였고, pAG67 및 pAG81 clone된 길항관련 유전자의 flanking sequence를 야생 길항균 Pseudomonas maltophilia B-14의 DNA를 probe로 사용하여 Southern hybridization으로 확인하였으며, 제한효소 지도를 작성하여 8Kb 및 4Kb 크기의 flanking sequence가 cloning되었음을 확인하였다. pAG6 및 pAG81의 flanking sequence를 EcoRi-BglII와 EcoRI-MpaI으로 분리하여 유전자 은행으로부터 길항관련 유전자가 cloning된 cosmid clone 7개주를 선발하였다.

Keywords

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