초록
Using the hematofluiorometer normal values of the erythrocyte zinc protoporphyrin(ZPP) and ZPP/Heme ratio were measured in 312 males and 163 females aged from 6 month to 73 years old and compared with those of anemic persons. The mean$\pm$SD values of ZPP of normal Koreans were 28.5$\pm$6.4($\mu\textrm{g}$/dl) in males and 3.18$\pm$7.7 in females. the mean$\pm$SD of ZPP/Heme ratio were 49.5$\pm$12.3($\mu$mol/mol heme) in males and 62.0$\pm$15.8 in females. The difference in the mean ZPP and ZPP/Heme ratio values between male and female subjects were statistically signficant(p<0.0001) In male subjects the mean ZPP and ZPP/Heme ratio of the age groups less than 15 years old were higher than adult groups and the difference between age groups was significant(p<0.005 and p<0.0001 respecti-vely) The normal upper limit of the mean$\pm$2SD in normal male and female subjects were 41.3 and 47.2 for ZPP and 74.1 and 93.6 for ZPP/Heme ratio respectively. The mean values of ZPP and ZPP/Heme ratio measured in the anemic persons were higher than those of normal subjects and did not show any significant difference by the sex and age groups except in 6-14 years male groups. The test specificity(positivity) analyzed in the anemic persons by the cut-off values calculated from the normal data were 50.6% for ZPP and 73.0% for ZPP/Heme ratio. The correlation analysis between blood hemoglobin and erythrocyte ZPP or ZPP/Heme ratio in the total 801 normal and anemic subjects showed that there are very high logarithmic correlation between the hemoglobin levels and ZPP/Heme ratio (r=-0.8339) and high correla-tion between the hemoglobin levels and ZPP concentrations(r=-0.6372) These results suggested that the measurement of the erythrocyte ZPP and ZPP/Heme ratio with the hematofluorometer can be a usuful screening method for iron deficiency anermin because they provide a reliable immediate results with a small amount of sample and are relatively simple and inexpensive.