Demonstration of Newcastle Disease Virus Antigens in Paraffin Embedded Tissues of Experimentally Infected Chickens Using Peroxidase-antiperoxidase(PAP) Technique

Peroxidase-antiperoxidaes법을 이용한 실험감염 계의 조직내 뉴캣슬병 바이러스 항원동정

This study was done to identify Newcastle disease virus(NDV) antigens in paraffin sections of various organs from experimentally NDV-infected chicken using peroxidase-antiperoxidase(PAP) technique. Sections were Incubated with rabbit anti-NDV polyclonal as first antibody, followed by incubation with goat anti-rabbit IgG conjugate and peroxidase anti-peroxidase ( PAP ). Positive reactions were often detected in the epithelim of trachea and in the lymphocyte of spleen at 24 hours after virus inoculation. The viral antigen was localized mainly in the cytoplasm of infected cells. The method approved to be highly specific for the indetification of NDV and allowed a precise localization of the viral antigens in infected cells.