The Culture Conditions of Mucor mucedo C-7 for Producing the Milk-Clotting Enzyme

응유효소 생산을 위한 Mucor mucedo C-7의 배양조건

  • 조재민 (충주공업전문대학 식품공업과) ;
  • 이웅수 (충주공업전문대학 식품공업과) ;
  • 김교창 (충북대학교 농과대학 식품공학과)
  • Published : 1992.08.01

Abstract

Mucor mucedo C-7 selected as a potent fungus for producing milk-clotting enzyme was cultured on wheat bran solid medium and the optimum culture conditions for the production of milk clotting enzyme were ot tamed as follows. Amount of water added to wheat bran was 100% to the weight of wheat bran and culture temperature and time was 3$0^{\circ}C$ and 72hrs, respectively. The production of milk-clotting enzyme was markedly increased by the addition of Macllvaine buffer solution (pH4.5) instead of water added to wheat bran solid medium and milk-clotting activity was stable for culture period.

효과적인 응유효소 생산을 위해 응유효소 생산 균주로 선정된 Mucor mucedo C-7을 밀기울 고체배지에 배양하여 최적 배양 조건을 검토하였다. 그 결과 밀기울에 대한 첨수량은 밀기울량에 100% 첨수하였을때 효소 생산이 가장 양호하였으며 배양 은도와 시간은 각각 3$0^{\circ}C$, 72시간이 가장 적당함을 알 수 있었다. 또 밀기울 배지에 증류수 대신 Macllvaine 완충용액 (pH4.5)을 첨가 하였을 경우 효소 생산은 현저히 증가하였으며 응유효소의 활성도 중류수를 첨수한 경우 보다 안정함을 알 수 있었다.

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