Continuous Production of Fructo-oligosaccharides by Immobilized Cells of Aureobasidium pullulans

Continuous production of fructo-oligosaccharides employing a packed bed reactor charged with immobilized cells of Aureobasidium pullulans was investigated. The optimum conditions for reactor operation were a feed concentration of 860 g/l; a feed rate, expressed as superficial space velocity of $0.2\;h^{-1}$, and a temperature of $50^\circ{C}$. Under these optimum conditions, the productivity of the reactor was $180\;g/l\cdot{h}$. Initial activity was maintained for more than 100 days. The reactor was successfully scaled up to a production scale of 1000l.