Antioxidative Effect of Some Edible Plant Solvent Extracts with Various Synergists

패모, 어성초, 쇠비름 및 들깨박 에탄올 추출물의 순차용매 분획별 항산화 효과

The antioxidative effect of the 75% ethanol extracts of preliminary selected Fritillaria ussuriensis Max(Fs), Houttuynia cordata Thunb(Hc), Portulaca oleracea L.(Po) and Perilla frutescene Var javanica Hara cake(Pf) were tested on palm oil and lard by rancimat test. Each solvent fraction of chloroform, ethyl acetate(EtOAc), butanol and water, was also evaluated its antioxidative effect with some synergists, like as ascorbic acid, citric acid and ${\delta}-tocopherol$. Po extract showed higher antioxidative effect on lard and the fraction of all test plants were the most effective on palm oil and lard with ascorbic acid and ${\delta}-tocopherol$. When 200 ppm of EtOAc fraction of Fs, He and Po extract each with 200 ppm of ascorbic acid were added to palm oil, the antioxidative index(AI, induction time of oil containing each extract/induction time of test oil) were 1.60, 1.53 and 1.47 respectively and 200 ppm of EtOAc fraction of Po and Pf extract each with 200 ppm of tocopherol to lard, the AI were 3.19 and 3.63 respectively.

예비 실험 결과 항산화 효과가 있는 패모, 어성초, 쇠비름 그리고 들깨박의 75%ethanol 추출물을 얻고 이를 다시 chloroform, ethyl acetate, butanol 그리고 물 순으로 용리, 분획물을 얻고 이들과 함께 tocopherol, citric acid, ascorbic acid를 팜유와 돈지에 첨가, Rancimat으로 항산화 효과를 비교하였다. 쇠비름 추출물이 돈지에 대한 산화 억제 효과가 가장 높았으며(AI 1.41)모든 실험 식물 추출물의 ethyl acetate 분획불에서 가장 높은 항산화성을 보였고 synergist로서는 ascorbic acid가 우수하였고 ${\delta}-tocopherol$을 혼합시에도 효과가 상승하였다. 패모, 어성초, 쇠비름의 ethyl acetate 분획층과 ascorbic acid 200ppm씩을 팜유에 첨가하였을 때 antioxidative index(AI)는 각각 1.60, 1.53 그리고 1.47이었고 쇠비름과 들깨박 ethyl acetate 분획층과 ${\delta}-tocopherol$ 200ppm씩을 돈지에 첨가했을 때 AI는 각각 3.19 및 3.63으로 높은 항산화 효과를 보였다.