Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Isolation and Characterization of Chloroplast DNA in Korea Ginseng, Panax gindeng C.A. Meyer

고려인삼(Panax ginseng C.A. Meyer)의 엽록체 DNA 분리 및 특성조사

  • Lee, Jeong-Heon (Korea Ginseng and Tobacco Research Institute, Science Town) ;
  • Lim, Yong-Pyo (Department of Horticulture, Chungnam National University) ;
  • Choi, Kwang-Tae (Korea Ginseng and Tobacco Research Institute, Science Town)
  • 이정헌 (한국인삼연초연구소 유전생리부) ;
  • 임용표 (충남대학교 농과대학 원예학과) ;
  • 최광태 (한국인삼연초연구소 유전생리부)
  • Published : 1993.04.01
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Abstract

In Korean ginseng, Panax ginseng C.A Meyer, it was difficult to isolate chloroplast DNA with classical methods, because of the high polysaccharide content of ginseng chloroplast The simple and efficient method of chloroplast DNA isolation from ginseng leaves has been developed by motificalion of recently advanced methods. Also, it can be successfully applied to ctDNA isolation of Chinese cabbage, radish, petunia tobacco as well as ginseng. Isolated chloroplast DNA from ginseng was digested with various restriction endonucleases. It was estimated that the molecular weight of Korean ginseng chloroplast DNA was about 142 kb. There was no difference in restriction endonuclease digestion patterns between two variants of Korean ginseng, which are Jakyung-Jong (violet-stem variant) and Hwang- sook-Jong (yellow-berry variant).

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