Journal of Oral Medicine and Pain
- Volume 18 Issue 1
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- Pages.73-82
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- 1993
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- 2288-9272(pISSN)
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- 2383-8493(eISSN)
Changes in the Oral Microflora in Patients with Acute Myeloid Leukemia during the Period of Induction Therapy
항암 화학요법중인 급성 골수성 백혈병 환자의 구강내 세균변화에 관한 연구
- Byul-Hee Lee (Dept. of Oral Medicine and Diagnosis, The Graduate School, Yonsei University) ;
- Chong-Youl Kim (Dept. of Oral Medicine and Diagnosis, The Graduate School, Yonsei University)
- Published : 1993.06.01
Abstract
To investigate the changes in aerobic and facultative anaerobic oral microflora during remission-induction chemotherapy in patients with acute myeloid leukemia, 10 consecutive patients were studied during a period of 28 days. One day before, during and after the induction therapy, patients were given 10% Betadine solution for mouthrinses after breakfast and kept from eating and drinking. After 3 hours, paraffin-stimulated whole saliva was obtained for 2 minutes and transported to the laboratory. The samples were dispersed and homogenized by use of vortex mixer for 20 seconds. From these samples 10-fold serial dilutions (from 10-1 through 10-3) were prepared. Each dilution of 0.1 ml was plated on duplicate set of one nonselective medium (Blood agar) and four selective media (Sabourauds dextrose agar, Mannitol salt agar, Mac-Conkey agar, SF medium ) using applicator woods. All agar plate were incubated at 37
Keywords
- remission induction chemotherapy;
- salivary flow rate;
- total number of microorganisms in saliva;
- acute myeloid leukemia