Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Studies on the Protoplast Fusion between Lactobacillus bulgaricus and Lactobacillus helveticus

Lactobacillus bulgaricus와 Lactobacillus helveticus간의 Protoplast 융합에 관한 연구

  • 박현정 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과) ;
  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 송재철 (울산대학교 자연과학대학 식품영양학과)
  • Published : 1993.04.01
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Abstract

The optimal conditions for protoplast fusion between the cells of kanamycin resistant Lactobacillus bulgaricus IFO 13593 and those of lincomycin resistant Lactobacillus helveticus IAM 12090 were investigated in this study. The highest fusion frequency of 9.1*10-4 was obtained when protoplast fusion was carried out for 0.5 min using 40% PEG 4000 solution as a cell fusion stimulant and subsequent post-PEG-incubation was undergone at 30 for 30 min in the PPI medium.

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References

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