한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제21권3호
  • /
  • Pages.214-220
  • /
  • 1993
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Saccharomyces cerevisiae를 이용한 온도조절형 발현 Vector의 개발

Construction of a Temperature Controlled Expression Ve e tor in Saccharumy ces cerevisiae

  • 최진옥 (경남대학교 공과대학 식품공학과) ;
  • 황용일 (경남대학교 공과대학 식품공학과)
  • 발행 : 1993.06.01
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초록

The mating type a of yeast, Saccharomyces cerevisiae mutant with hmla2-102 and sir3-8ts was changed to type alpha by changing the growth temperature from 25C to 35C. A temperature-sensitive expression vector system was constructed using mating factor alpha1 (Mfalpha1) gene encoding alpha factor which is expressed in the type alpha cells. Vectors with different copy numbers were constructed by joining the promoter and pre or prepro-secretion single sequence of Mfalpha1 to promoterless PHO5' gene as a reporter gene.

키워드

참고문헌

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