Construction of the Recombinant phbCAB Operon of Alcaligenes eutvtrphus for Accumulation of Poly-$\beta$-hydroxybu tyric Acid in Escherichia coli

Alcaligenes eutrophus phbCAB Operon의 재조합과 Poly-$\beta$-hydroxybutyric Aicd의 대장균내 축적

  • 김경태 (경북대학교 자연과학대학 유전공학과) ;
  • 박진서 (경북대학교 자연과학대학 유전공학과) ;
  • 이용현 (경북대학교 자연과학대학 유전공학과) ;
  • 허태린 (경북대학교 자연과학대학 유전공학과) ;
  • 박해철 (경북대학교 자연과학대학 유전공학과)
  • Published : 1993.06.01

Abstract

In order to achieve poly-beta-hydroxybutyric acid (PHB) production using recombinant DNA in various host bacterial cells, the isolation of genes for PHB biosynthesis was attempted. As a result, a 5.2kb DNA fragment containing phbCAB operon of Alcaligenes eutrophus was isolated by colony hybridization using synthetic oligodeoxyribonucleotides as probes. The constructed recmbinant plasmid pSK(+)-phbCAB operon was transferred to Escherichia coli, and the obtained transformant accumulated considerable amount of PHB.

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