Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Media Optimization and Fed-Batch Fermentation for Riboflavin Overproduction by Ashbya gossypii

Ashbya gossypii로부터 riboflavin 대량생산을 위한 배지 최적화와 유가식 배양

  • 남수완 (한국과학기술연구원 유전공학연구소) ;
  • 장형욱 (한국과학기술연구원 유전공학연구소) ;
  • 반재구 (한국과학기술연구원 유전공학연구소) ;
  • 민태익 (한국과학기술연구원 유전공학연구소) ;
  • 김익환 (한국과학기술연구원 유전공학연구소)
  • Published : 1993.12.01
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Abstract

In order to maximize the riboflavin production by a mutant strain Ashbya gosspyii, the optimization of medium and fed-batch fermentation were performed. As carbon sources, glucose and soybean oil were necessary for the riboflavin overproduction. Optimal concentrations of glucose and soybean oil in the flask cultures were found to be 3.0% and 0.5%, respectively, in a complex medium containing corn steep liquor(CLS) 1%. Among the various organic nitrogen sources tested, CSL was the most effective one both for the cell growth and riboflavin overproduction.

Keywords

References

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