Studies on the Chemical Components and Biological Activities of Edible Plants in Korea (II) - Isolation and Quantitative Analysis of Flavonoids from the Leaves of Cedrela sinensis A. Juss. by HPLC -

한국산 식용식물의 화학성분 및 생리활성에 관한 연구 (II) - HPLC에 의한 참죽나무 잎중 Flavonoid 성분의 확인 및 정량 -

  • Published : 1993.10.01

Abstract

Five flavonoids isolated from the ethyl acetate fraction of Cedrela sinensis A. Juss. were identified by high performance liquid chromatography. Separation was achieved by reversed phase chromatography on ${\mu}-bondapak$ C18 column with isocratic elution method. The content of the major flavonoid, quercitrin was about 9.48%(w/w) and 37.06%(w/w) for the methanol extract and ethyl acetate fraction, respectively.

참죽나무(Cedrela sinensis A. Juss.) 잎의 ethyl acetate분획으로부터 분리한 5종의 flavonoid성분들은 HPLC에 의한 용매계 THF-dioxane-MeOH-HOAc-5% $H_3PO_4-H_2O$(145 : 125 : 50 : 20 : 2 : 658)에서 양호한 분리능을 나타내었다. 또한 주성분인 quercitrin의 함량은 MeOH엑스 및 EtOAc 분획에서 각각 9.48%(w/w) 및 37.06%(w/w) 함유되어 있음을 알 수 있었다.

Keywords

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