Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Changes in the Enzyme Activities, Pectins and Structure of Persimmon Fruit during Softening

감과실의 연화중 효소활성, 펙틴 및 조직의 변화

  • Published : 1993.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate change in the polygalacturonase and ${\beta}-galactosidase$ activities, pectins, cell wall structure of persimmon fruit during ripening and softening. Polygalacturonase and ${\beta}-galactosidase$ activities were not detected at turning stage. However polygalacturonase activities of mature and soft persimmon fruits were 55.01 and 206.70units/100g-fresh weight(fr. wt.), respectively. ${\beta}-Galactosidase$ activities of mature and soft persimmon fruits were 21.79 and 380.23unit/100g-fr. wt. respectively. The contents of total and insoluble pectins increased during maturation but decreased during softening. The content of water-soluble pectin increased during maturation and softening. The intercellular space was in larged during ripening, and middle lamella was degraded in mature persimmon fruit, and the cells of soft persimmon fruit were separated each other.

본 연구는 감과실의 성숙과 연화중에 일어나는 세포벽분해효소의 활성, 펙틴 및 조직의 변화를 조사하였다. Polygalacturonase와 ${\beta}-galactosidase$의 활성은 미숙과실에 없었으나 완숙 감과실에서 각각 55.1, 21.79unit/100g-fr. wt.이었고 연시에서는 각각 206.70, 380.23unit/100g-fr, wt으로 증가하였다. 펙틴질의 변화는 총 펙틴과 불용성 펙틴은 성숙중에 증가하였으나 연시에서는 현저히 감소하였으며, 반면에 수용성 펙틴은 성숙과 연화중에 증가하였다. 감과실의 성숙주엥 간극의 발달과 더불어 middel lamella의 용해현상이 일어났으며 연시에서는 세포가 분리되었다.

Keywords

References

  1. 식품카르테 유태종
  2. Horticutural Reviews v.5 The role of cell wall hydrolases in fruit softening Huber,D.J.
  3. Recent advances in the biochemistry of fruits and vegetables Enzymes and lexture changes during ripening Hobson,G.E.;Friend,J.(ed.);Rhodes,M.J.C.(ed.)
  4. Planta v.155 Synthesis of polygalacturonase during tomato fruit ripening Tucker,G.A.;Grierson,D.
  5. J. Food. Sci. v.33 Cellulase activity during maturation and ripening of tomato fruit Hobson,G.E.
  6. Plant Physiol v.61 Cellulase activity and fruit sofening in avocado Pess,G.E.;Fuchs.Y.;Zauberman;G.Cellulase
  7. J. Sci. Food Agr. v.33 Purification and changes in activities of tomato pectinesterase isoenzymes Tucker,G.A.;Robertson,N.G.;Grierson,D.
  8. Recent advances in the biochemistry of fruits and vegetables Composition and metabolism of cell wall polysaccharldes in ripening fruits Knee,M;Bartely,I.M.;Friend,J.(ed.);Rhodes,M.I.C.(ed.)
  9. Phytochemistry v.23 no.11 Changes in cell wall neutral sugar composition during fruit ripening Gross,K.C.;Sams,C.E.
  10. Plant Physiol v.64 Ultrastructural changes in the cell wall of ripening apple and pear fruits Ben-Arie,R.;Kislev,N.;Frenkel,C.
  11. Plant Physiol v.71 β-Calactosiase in ripening tomatoes Pressey,R.
  12. J. Food Biochem v.8 A rapid and sensitive spectrophotometric method for assaying polygalacturonase using 2-cyanoacetamide Gross,K.C.
  13. J. Amer. Soc. Hort. Sci. v.104 no.4 Metabolism of the pectic substances in ripening pears Ben-Arie,R.;Sonego,L.;Frenkel,C.
  14. Anal. Biochem v.1 A modified uronic acid carbazole reaction Bitter,T.;Muir,H.M.
  15. J. Biophys. Biochem v.62 Improvements in epoxy resin embeding methods Luft,J.H.
  16. J. Food Sci. v.36 Development of polygalacturonase activity and solubilization of pectin in peaches during ripening Pressey,R.;Histon,D.M.;Avants,J.K.
  17. Plant Physiol v.142 Ultrastructure of tomato fruit ripening and the role of polygalacturonase isoenzymes in cell wall degradation Crookes,P.R.;Cierson,D.
  18. Phytochemistry v.13 β-Galactosidase activity in ripening apple Bartley,I.M.
  19. J. Exper. Bot. v.28 A further study of β-galactosidase activity in apple ripening in storage Bartley,I.M.
  20. Phytochemistry v.12 Polysaccharide changes in cell walls of ripening apples Knee,M.
  21. 한국식품과학회지 v.22 no.7 감과실의 성숙과 추숙증의 세포벽 다당류의 비섬유성 단당류의 변화 신승렬;김순동;송준희;김광수
  22. J. Food. Sci. v.36 Textural changes and molecular characteristics of pection constituent in ripening peaches Shewfelf,A.L.;Payter,V.A.;Jen,J.J.