Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

The Effect of Alcohol Administration on Selenium Concentration and Cell Morphology of Heat and Liver of Rats Fed with the Different Levels of Selenium and Vitamin E

알코올의 섭취가 식이내 Selenium과 Vitamin E 수준을 달리한 흰쥐의 체내 Selenium 수준과 심장 및 간조직에 미치는 영향

  • 김갑순 (경남전문대학 식품영양과) ;
  • 채기수 (경남전문대학 식품영양과) ;
  • 정승용 (경상대학교 식품영양학과)
  • Published : 1993.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the effect of alcohol administration on selenium concentration and cell morphology in tissurs of rats fed with the different levels of selenium (Se) and vitamin E. Seventy two male rats of Sprague-Dawley strain weighing about 58~62g were divided into 12 groups. The dietary Se levels were 0mg(L-), 0.4mg(C-) and 10mg(H-), and the dietary vitamin E levels were 0mg(-L) and 150mg(-C) per kg diet, respectively. Alcohol-adminstrated groups(--A) received the triple distilled potable water solution containing 10% of ethanol from the 3rd week of experimental periods. The obtained experimental results are summarized as follows. Se concentration in blood and urine made difference in accordance with Se level in diet and tended to be low in alcohol administrated groups. Se concentration in liver and kidney was also directly proportional to the dietary Se level, and it tended to be low in each alcohol group, but Se concentration in kidney tended to be increased by alcohol administration. Myocardium in rats showed lysosome increasing, fat droplet, mitochondrial swelling, and in particular, bad intracellular edema, in H-group fed with high Se and in L-group with low Se. It also showed such phenomena in the alcohol administrated group. In HC-group fed with excessive Se and normal vitamin E. there appeared no noticeable change in liver tissue. However, in the alcohol administrated HCA-group, there came out fat droplet. Especially, in the alcohol administrated LLA-group, not fed with sufficient Se and vitamin, E, there were found lysosome increasing and a number of fat droplet.

알코올의 섭취가 Se가 vitamin E 수준을 달리한 흰주의 체내 Se 수준 및 심장, 간세포 형태에 미치는 영향을 살펴보기 위하여 평균 체중이 58~62g인 Sprague-Dawley계의 젖을 갖뗀 숫쥐 72마리를 12군으로 나누어 Se의 수준을 식이 kg당 0mg, 0.4mg,10mg으로 달리하고 vitamin E는 식이 Kg당 0mg, 150mg으로 하여 7주간 사육시켰다. 알코올 섭취는 실험시작 3주째부터 ethanol을 급수물에 10% 맞추어 섭취군과 비섭취군으로 나누어서 섭취군에서는 제한없이 먹게 하였다. 얻어진 결과를 요약하면 다음과 같다. 혈액과 소변의 Se의 수준은 식이내 Se 수준의 차이에 따라 유의적인 차이를보여 식이내 Se수준이 높은 H-군, 정상적으로 섭취한 C-군, 결핍된 L-군의 순으로 높았으며 알코올 섭취군들은 낮은 경향을 보였다. 간장, 신장의 Se의 농도는 식이 Se 수준에 비례하여 H-, C-, L- 순으로 높았으며 알코올 섭취로 인한 Se농도의 변화는, 알코올을 섭취한 군둘의 Se 농도가 낮은 경향이나 신장에서는 반대로 높은 경향이었다. 전자현미경을 통한 관찰에서 심근세포 조직형태의 변화는 식이내 Se수준이 높은 H-군들, Se수준이 낮은 L-군에서 lysosome의 증가 및 지방적 출현, mitochondria의 종창, 특히 모세혈관의 내피세포에 심한 종창이 일어났고, 알코올을 섭취한 군들에도 심근세포의 종창, lysoscme의 증가, mitochondria 변화를 보였다. Se을 과잉으로 섭취하고 vitamin E를 정상으로 섭취한군(HC-)에서는 특이한 변화가 없었으나 알코올을 투여한 HCA군에서 지방적이 나타났다. 특히 Se과 vitamin E이 결핍되고 알코올을 섭취한 LLA군에서는 lysosome의 증가 및 지방적이 많이 증가 하였다.

Keywords

References

  1. Science v.179 Selenium : Biochemical role as a component of glutathione peroxidase Rotruck,J.T.;Pope,A.L.;Gonter,H.E.;Hafeman,D.G.;Hoekstra,W.G.
  2. J. Nutr. v.107 Lipid peroxidation in vivo during vitamin E and selenium deficiency in the rat as monitored by ethane evolution Hafemn,D.G.;Hoekstra,W.G.
  3. Vit. Horm. v.20 Vitamin E as biological lipid antioxidant Tappel,A.L.
  4. J. Nutr. v.104 Effect of dietary selenium on glutathione peroxidase in the chick Omaye,S.T.;Tappel,A.L.
  5. Fiber Lesions Laboratary investigation v.37 Ultrastructural alterations in nutritional cardiomyopathy of selenium vitamin E deficient swineⅠ Van Vleet,J.F.;Ferrans,V.J.;Ruth,G.R.
  6. J. Nutr. v.120 Dietary supplements of vitamin E, β-carotene, coenzyme $Q_{10}$ and selenium protect tissues against lipid peroxidation in rat tissue slices Veibovitz,B.;Hu,M.;Tappel,A.L.
  7. Science v.162 Ethanol oxidation by hepatic microsomes-adaptive increase after ethanol feeding Lieber,C.S.;Decarli,L.M.
  8. Ewah Med. v.7 The effect of vitamin A and E on lipid peroxidation in ethanol administered rat liver microsomes Hong,Y.S.;Ham,Y.A.;Sung,N.E.
  9. Am. J. Clin. Nutr. v.38 Selenium and acute alcoholism Dutta,S.K.;Miller,P.A.;Greenberg,L.B.;Levander,O.A.
  10. Alcohol v.23 Alcohol intake correlated with serum trace elements Karkkainen,P.;Mussalo-Rauhamaa,H.;Poikolainen,K.;Lehto,J.
  11. Brit. J. Nutr. v.55 Selenium status in patients with liver cirrhosis and alcoholism Johansson,U.;Johansson,F.;Joelsson,B.;Brglund,M.;Akesson,B.
  12. Am. J. Clin. Nutr. v.38 Selenium and acute alcoholism Dutta,S.K.;Miller,P.A.;Greenberg,L.B.;Levander,O.A.
  13. 한국식품영양학회지 v.4 no.1 식이내 Se수준과 알콜섭취가 흰쥐의 지질과산화와 지방대사에 미치는 영향 김갑순;김민식;김두진;채기수
  14. 한국영양식량학회지 v.22 no.2 식이내 selenium과 vitamin E가 alcohol을 섭취한 흰쥐의 간지질과산화에 관련된 효소의 활성에 미치는 영향 김갑순;정승용;김석환
  15. J. Anal. Atmoc Spect. v.2 Direct electrothermal alomisation atomic absorption spectrometric determination of selenium in whole blood and serum with continuum-source background correction Saed,K.
  16. J. Biophys. Biochem. Cytol. v.9 Improvement on epoxyrein embedding method Luft,J.H.
  17. J. Cell Biol. v.7 The use of lead citrate at high pH as an electron opaque stain in electron microscopy Reynolds,E.S.
  18. Ann. Nutr. Metab. v.31 Effect of various dietary selenium intakes on the levels of blood glutathione-peroxidase and selenium in long-term fed rats Testolin,G.;Ciappellano,S.;Lanzola,E.;Allegrini,M.
  19. J. Clin. Invest. v.65 Liver necrosis and lipid peroxidation in the rat as the result of paraquat and diquat administraiton Burk,R.F.;Laurence,R.A.;Lane,J.M.
  20. Arrestees v.20 Decreased serum selenium and magnesium levels in drunkeness Mussalo,R.H.;Piokolainen,K.;Karkkainen,P.P.;Lehto,J.
  21. N. Engl. J. Med. v.303 Decreased serum selenium in alcoholic cirrhosis Aaseth,J.;Thomassen,Y.;Alexander,J.;Norheim,G.
  22. J. Nutr. v.118 Effect of selenium and vitamin E status on plasma creatine kinase activity in claves Arthur,J.R.
  23. J. Nutr. v.95 Tissue selenium levels during the development of dietary liver necrosis in rats fed torula yeast diets Burk,R.F.;Whitney,R.;Frank,H.;Pearson,W.N.
  24. Am. J. Clin. Nutr. v.42 Selenium intake and metabolic balance of 10 men from a low selenium area of China Luo,X.M.;Wei,H.J.;Yang,C.L.;Xing,J.;Qiao,C.H.;Feng,Y.;Liu,J.;Liu,Q.;Wu,Q.;Liu,Y.X.;Stoecker,B.;Spallholz,J.E.;Yang,S.P.
  25. Am. J. Clin. Nutr. v.34 Selenium balance in young men during selenium depletion and repletion Levander,O.A.;Sutherland,B.;Morris,V.C.;King,J.C.
  26. Am. J. Clin. Nutr. v.39 Dietary selenium levels needed to maintain balance in North American adults consuming self selected diets Levander,O.A.;Morris,V.C.
  27. J. Nutr. v.118 Effect of various dietary levels of selenium as selenite or selenomethionine on tissue selenium levels and glutathione peroxidase activity in rats Whanger,P.D.;Butler,J.
  28. J. Nutr. v.116 The response of selenium-deficient mice to Candida ablicans infection Boyne,R.;Arthur,J.R.
  29. Br. J. Nutr. v.55 Some effects of selenium deficiency on glutathione peroxidase activity and tissue pathology in rainbow trout(Salmo gairdneri) Bell,J.;Pirie,N.J.S.;Adron,J.W.;Cowey,C.B.
  30. Chin. Med. J. v.92 Observations on effect of sodium selenite in prevention of Keshan disease Keshan Diease Research Group