Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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The Bleaching Effects of Potato Lipoxygenase Isoenzymes on ${\beta}-Carotene$

감자 Lipoxygenase Isoenzymes의 베타-카로텐 탈색효과

  • 문정원 (부산여자전문대학 가정과) ;
  • 조순영 (강릉대학교 식품과학과) ;
  • 서명자 (부산대학교 식품영양학과)
  • Published : 1993.12.01
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Abstract

The bleaching effect of potato lipoxygenase Isoenzymes on ${\beta}-carotene$ was studied. Two lipoxygenase Isoenzymes(LOX-1, LOX-2) from potato tuber were purified by CM-cellulose, DEAE-cellulose ion exchange chromatography. LOX-1 and LOX-2 seemed to have bleaching effect on ${\beta}-carotene$ in the presence of linoleic acid, which the decrease in the formation of conjugated dienes. LOX-2 was founded to have a greater pigment bleaching activity than that of LOX-1.

황산암모늄분획침전, CM-cellulose, DEAE-cellulose 이온교환크로마토그라피를 이용하여 감자에서 2종의 lipoxygenase isoenzymes(LOX-1, LOX-2)을 분리, 정제하여 isoenzyme 각각의 베타-카로텐에 대한 탈색능을 실험하였다. LOX-1, LOX-2는 linoleic acid 존재하에서 베타-카로텐에 대해 탈색효과를 나타냈으며 탈색효과는 공역산의 감소와 더불어 일어났다. 베타-카로텐 탈색시 공역산의 감소는 LOX-2에 비해 LOX-1이 더 컸으나 탈색효과는 LOX-2가 더 높았다.

Keywords

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