Properties of the Extracellular Proteins Produced by Bacillus sp. WY-60

Bacillus sp. WY-60이 생산한 균체외 단백질의 특성

  • Kwon, Oh-Jin (Dept. of Food Science and Technology, Yeungnam University) ;
  • Park, Shin (Dept. of Agricultural Chemisty, Taegu University)
  • 권오진 (영남대학교 식품가공학과) ;
  • 박신 (대구대학교 농화학과)
  • Published : 1993.12.01

Abstract

Extracellular proteins of Bacillus sp. WY-60 were obtained, and then the properties of the isolated proteins were characterized. The proteins were composed of two kinds of protein in size. The molecular weight of the major protein was around 21,000 according to the gel filtration chromatography and SDS-polyacryamide gel electrophoresis. The amino acid composition showed that glutamic acid was a major amino acid with the concentration of 26.16mg/g. The isoelectric point of the proteins was about pH 7.5.

Keywords

References

  1. Agric. Biol. Chem. v.40 no.3 Extracellular production of proteins by microorganisms. I. Screening for protein producing bacteria Udaka,S.
  2. Agric. Biol. Chem. v.44 no.1 Cultural conditions for protein production by Bacillus brevis No. 47 Shaku,M.;Koike,S.;Udaka,S.
  3. Agric. Biol. Chem. v.44 no.10 Protein production in chemically defined medium by Bacillus brevis No. 47 Tsuchida,T.;Miyashiro,S.;Enei,H.;Udaka,S.
  4. Agric. Biol. Chem. v.44 no.1 Effect of glycine and L-isoleucine on protein production by Bacillus brevis No. 47 Miyashiro,S.;Enei,H.;Hirose,Y.;Udaka,S.
  5. Appl. Environ. Microbiol. v.42 no.2 Effects of phosphate in medium on protein secretion in a protein-producing bacterium, Bacillus brevis 47 Tsukagoshi,N.;Yamada,H.;Tsuboi,A.;Udaka,S.
  6. J. Bacteriol. v.256 Morphological alterations fo cell wall concomitant with protein release in a protein-producing bacterium, Bacillus brevis 47 Yamada,H.;Tsukagashi,N.;Udaka,S.
  7. Agric. Biol. Chem. v.44 no.10 Stimulatory effect of inhibitors of cell wall synthesis on protein production by Bacillus brevis Miyashiro,S.;Enei,H.;Takinami,K.;Hirose,Y.;Tsuchida,T.;Udaka,S.
  8. 化學と生物 v.21 no.10 山根國男
  9. J. Biochem. v.94 Major proteins released by a protein-producing bacterium, Bacillus brevis 47, are derived from cell wall protein Ohmlzu,H.;Sasaki,T.;Tsukagoshi,N.;Udaka,S.;Kaneda,N.;Yagi,K.
  10. 한국농화학회지 v.36 no.1 Bacillus sp. WY-60에 의한 균체의 단백질의 분비조건 박신;권오진
  11. J. Biol. Chem. v.244 no.16 The reliability of molecular weight determination sodium dodesyl sulfate-polyacrylamide gel electrophotesis Weber,K.;Osborn,M.
  12. Proc. 3rd FAAP Symp. on protein metabolism and nutrition v.1 Mason.V.C.;Anderson,S.B.;Rodemo,M.
  13. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  14. 한국농화학회지 v.31 no.2 Bacillus sp. T₂-3가 생산한 균체의 단백질의 성질 이재숙;김찬조;이종수
  15. Report of joint FAO/WHO Expert Group on Protein Requirements FAO Nutrition Meetings, Report Series no.37 FAO/UN