초록
Lysozyme, glycine, EDTA의 혼합첨가가 김치발효에 미치는 영향을 고찰하였다 숙성기간별로 pH 4.0에서 pH 5.0까지 김치액에 각각 120ppm lysozyme과 0.12% glycine을 첨가하였을 때 첨가구가 $10^1$~$10^4$ cfu/$m\ell$만큼 젖산균의 성장이 억제되었고 lysozyme단독 첨가구에 비해 lysozyme-glycine 혼합첨가구가 $10^1$~$10^2$cfu/$m\ell$ 만큼 더 많은 젖산균의 성장이 억제되었다. Lysozyme 단독으로 100, 200, 1,000ppm을 배추김치에 첨가하면 대조구에 비해 발효기간에 따라 pH의 감소는 둔화되었지만 말기에는 처리구간 현저한 차이는 없었다. 따라서 lysozyme-glycine 혼합 첨가하므로써 적정산도와 pH 변화가 지연되었다. 120ppm과 2mM EDTA로 나누어 첨가한 김치에 대해서도 단독구에 비해 혼합첨가구에서 $10^1$~$10^2$cfu/$m\ell$의 젖산균이 억제되었다.
Effects of addition of 120ppm lysozyme and 0.12% glycine into Kimchi liquid on the growth of lactic acid bacteria (LAB) were investigated. The results showed that the growth of LAB was significantly reduced as much as 10$^1$~10$^4$cfu/$m\ell$ for the liquid having pH 5.5 during storage at 32$^{\circ}C$ while the liquid of pH 4.4 showed a reduction of LAB growth by 10$^1$~10$^2$cfu/$m\ell$. Combined addition of lysozyme and glycine was found to be more effective than those of individual additions. The decrease in pH of Kimrhi added with 100, 200 and 1, 000ppm lysozyme was slower than that of control, but there was no significant pH difference at late stage of fermentation. However Kimchi added with 100ppm lysozyme and 1% glycine decreased in the rate of pH change. Change in titratable acidity was also slowed up for Kimchi added lysozyme and glycine. The growth of LAB could be inhibited as much as 10$^1$to 10$^2$cfu/$m\ell$ in the Kimrhi sample added with 120ppm lysozyme and 2mM EDTA compared with other treatments including control.
