Journal of Microbiology and Biotechnology
- 제3권4호
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- Pages.261-265
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- 1993
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Localization of Sop Proteins and Interaction of Plasmid DNA with the Cell Membrane of Host Bacteria in Partitioning
- Kim, Sung-Uk (Bioproducts R.G., Genetic Engineering Research Institute, KIST) ;
- Nagai, Kazuo (Dept. of Bioengineering, Tokyo Institute of Technology) ;
- Tamura, Gakuzo (Dept. of Agricultural Chemistry, The Univ. of Tokyo,) ;
- Yu, Ju-Hyun (Dept. of Food Engineering, Yonsei Univ.)
- 발행 : 1993.12.01
초록
A sopA protein (41K) encoded by plasmid pXX288 was observed in the cytoplasm, whereas a sopB protein (37K) encoded by plasmid pXX157 was observed in the membrane fraction. Most of the sopB protein was solubilized from the crude membrane by treatment with Sarkosyl, which suggested that the protein may be located in the inner membrane. The sopA protein was precipitated at the concentration of 30 to 60% ammonium sulfate. The sedimentation profile of the crude membrane fraction showed a little difference according to culture media used, and the sopB protein existed in all fractions of inner membrane. The DNA of plasmids, pXX157, pXX300, and pXX167 co-sedimented with inner membrane fraction.