Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Partial Assignment of Heme Groups of Cytochrome $c_3$ of Desulfovibrio vulgaris Miyazaki F by $^{1}H$-NMR

  • Jang-Su Park (Department of Chemistry, College of Natural Sciences, Pusan National University) ;
  • Shin Won Kang (Department of Chemistry, College of Natural Sciences, Pusan National University)
  • Published : 1993.10.20
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Abstract

The $^1H-NMR$ signals of the heme methyl, propionate and related chemical groups of cytochrome $c_3$ from Desulfovibrio vulgaris Miyazaki F (D.v. MF) were site-specifically assigned by means of 1D-NOE, 2D-DQFCOSY and 2D-TOCSY spectra. They were consistent with the site-specific assignments of the hemes with the highest and second-lowest redox potentials reported by Fan et al. (Biochemistry, 29, 2257-2263 1990). The site-specific heme assignments were also supported by NOE between the methyl groups of these hemes and the side chain of Val-18.

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