Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Use of ELISA for the Residue Analysis of Pesticides

ELISA 기법을 이용한 농약(農藥)의 잔류분석(殘留分析)

  • Lee, Kang-Bong (Dept. of Agricultural Chemistry, College of Agriculture, Chonnam National University) ;
  • Suh, Yong-Tack (Dept. of Agricultural Chemistry, College of Agriculture, Chonnam National University)
  • 이강봉 (전남대학교 농과대학 농화학과) ;
  • 서용택 (전남대학교 농과대학 농화학과)
  • Published : 1993.12.30
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Abstract

Immunochemical assay, ELISA for small molecules such as pesticides are rapid, sensitive, cost effective and can easily analyze with large samples. ELISA is one of several powerful biotechnologies immediately applicable to pesticide analysis. This review lists the advantages and disadvantages of the ELISA and elucidate the steps in assay development using examples from this laboratory. The focus is primarily on hapten synthesis strategies, protein conjugation, Immunization, assay format, and assay validation.

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