대한수의학회지 (Korean Journal of Veterinary Research)
- 제33권1호
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- Pages.131-135
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- 1993
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
축우 부루셀라병의 ELISA 진단법에 관한 연구
Enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus
- 임윤규 (제주대학교 수의학과) ;
- 이두식 (제주대학교 수의학과) ;
- 박전홍 (제주대학교 수의학과) ;
- 양기천 (제주대학교 수의학과) ;
- 김승호 (제주대학교 수의학과) ;
- 김공식 (제주도가축위생시험소) ;
- 현관종 (제주도가축위생시험소) ;
- 김우택 (제주도가축위생시험소) ;
- 이영순 (서울대학교 수의과대학)
- Lim, Yoon-kyu (Department of Veterinary Medicine, Cheju National University) ;
- Lee, Doo-sick (Department of Veterinary Medicine, Cheju National University) ;
- Park, Jun-hong (Department of Veterinary Medicine, Cheju National University) ;
- Yang, Ki-chun (Department of Veterinary Medicine, Cheju National University) ;
- Kim, Seung-ho (Department of Veterinary Medicine, Cheju National University) ;
- Kim, Kong-sick (Cheju Veterinary Service Laboratory) ;
- Hyun, Kwan-jong (Cheju Veterinary Service Laboratory) ;
- Kim, Woo-tack (Cheju Veterinary Service Laboratory) ;
- Lee, Yong-soon (CoIlege of Veterinary Medicine, Seoul National University)
- 투고 : 1992.09.25
- 발행 : 1993.01.30
초록
Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were
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