THE CYTOTOXIC EFFECTS OF ROOT CANAL SEALERS ON FIBROBLASTS IN HUMAN PERIODONTAL LIGAMENT

근관 충전용 시멘트의 치주인대 섬유아세포 독성에 관한 연구

  • Lee, Gwang-Hee (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
  • Choi, Ho-Young (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
  • Min, Byung-Soon (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
  • Park, Sang-Jin (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
  • Choi, Gi-Woon (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University)
  • 이광희 (경희대학교 치과대학 보존학교실) ;
  • 최호영 (경희대학교 치과대학 보존학교실) ;
  • 민병순 (경희대학교 치과대학 보존학교실) ;
  • 박상진 (경희대학교 치과대학 보존학교실) ;
  • 최기운 (경희대학교 치과대학 보존학교실)
  • Published : 1993.04.25

Abstract

The purpose of this study was to evaluate for the cytotoxicity of root canal sealers - Tubliseal, N2, AH26, and Sealapex - on fibroblasts cultivated from human periodontal ligament. Succinate dehydrogenase activity test and $^{51}Cr$ release test were performed to evaluate the shortterm cytotoxicity. According to l,3 and 6 days vital cell count and $^{14}C$-leucine incorporation rate to fibroblasts were evaluated. The results of this study were as follows: 1. In the test of SDH activity by millipore filter method, Sealapex showed mild cytotoxicity but Tubliseal, N2 and AH26 showed severe cytotoxicity. 2. In $^{51}Cr$ release test, Tubliseal was the most cytotoxic sealer tested, and rank ordered the relative cytotoxicity of the other sealers tested as follows: AH26, N2, Sealapex. 3. In the test of viable cell count, cytotoxicity in Tubliseal was continued because vital cell number reduced with time. Because vital cell was not showed in N2 and AH26 at 1 day, it was recognized that N2 and AH26 exhibited severe cytotoxicity. In Sealapex, vital cell number increased remarkably with time, so it showed that cytotoxicity decreased with time. 4. In $^{14}C$-leucine incorporation rate test, protein sythesis was not produced in Tubliseal, N2 and AH26 after 3 days, it showed that cytotoxicity in Tubliseal, N2 and AH26 was severe. Although protein synthesis in Sealapex decreased with time, it continued after 6 days. Therefore Sealapex has been exhibited mild cytotoxicity.

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