The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Correlative Production of Mn-Peroxidase and Glucose Oxidase Depending on the Culture Condition of Schizopora paradoxa

좀구멍버섯균의 배양조건에 따른 Mn-Peroxidase와 Glucose Oxidase의 생성조절

  • Lee, Sang-Yoon (Department of Biology and Department of Horticulture Kangnung National University) ;
  • Shin, Hyeon-Dong (Department of Biology and Department of Horticulture Kangnung National University) ;
  • Kim, Kyu-Joong (Department of Biology and Department of Horticulture Kangnung National University)
  • 이상윤 (강릉대학교 생물학과,원예학과) ;
  • 신현동 (강릉대학교 생물학과,원예학과) ;
  • 김규중 (강릉대학교 생물학과,원예학과)
  • Published : 1994.12.30
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Abstract

White-rot fungus, Schizopora paradoxa did not produce Mn-peroxidase and glucose oxidase without manganese. But, in high concentration of manganese (40 ppm), the activities of both enzymes were higher than those in basal concentration of manganese (11.15 ppm). Unlike the activities of the enzymes, mycelial mass was the same level as the control culture (11.15 ppm manganese) through out the culture period, depending on the concentration of manganese. The same experiments were carried out for the effect of copper and veratryl alcohol added to the culture. The results were not consistent dependent on the concentration of copper and veratryl alcohol, respectively. The involvement of cAMP in the correlative production of MNP and GOX was investigated. In this study, addition of atropine to the culture resulted in a concomitant inhibition of production of MNP and GOX, depending on the concentration of inhibitor added.

좀구멍버섯균(Schizopora paradoxa)은 백색 부후 균으로 망간이 배지에 첨가되지 않으면 MNP와 GOX는 동시에 생성되지 않았으며 고농도의 망간 첨가시 (40ppm)에는 기본적 농도(11.15ppm)에 비해 MNP와 GOX가 모두 높은 활성을 보였다. 그러나 망간농도에 따른 균사생장량에는 별 차이가 없었다. 구리와 veratryl alcohol에 대해서도 같은 실험을 실시하였으나 농도에 따른 MNP와 GOX의 변화가 상관관계를 갖지 않는 것으로 나타났다. MNP와 GOX의 생성조절과 관련하여 cAMP의 영향을 조사한 결과 cAMP생성효소 억제제인 atropine을 농도별로 배양액에 첨가시 정도의 차이는 있었으나 MNP와 GOX생성이 동시에 억제되었다.

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