The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Polygalacturonase from Ganoderma lucidum

Ganoderma lucidum으로부터 Polygalacturonase의 생산

  • Yoon, Sook (Department of Food Science & Technology, Chonbuk National University) ;
  • Kim, Myung-Kon (Department of Food Science & Technology, Chonbuk National University) ;
  • Hong, Jai-Sik (Department of Food Science & Technology, Chonbuk National University) ;
  • Kim, Myeong-Sook (Department of Food Science & Technology, Chonbuk National University)
  • 윤숙 (전북대학교 농과대학 식품공학과) ;
  • 김명곤 (전북대학교 농과대학 식품공학과) ;
  • 홍재식 (전북대학교 농과대학 식품공학과) ;
  • 김명숙 (전북대학교 농과대학 식품공학과)
  • Published : 1994.12.30
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Abstract

The optimum nutritional and cultural conditions of polygalacturonase by Ganoderma lucidum in liquid culture were studied. The optimal temperature, pH, and the duration of culture for production of the enzyme was $30^{\circ}C$, 5.5 and 14 days, respectively. The maximal production of the enzyme was obtained in a synthetic medium containing 10 g of pectin, 10 g of soluble starch, 1 g of yeast extract, 2 g of peptone, 1 g of phenylalanine, 2 g of $KH_2PO_4$, 0.2 g of $MgSO_4{\cdot}7H_2O$, 0.05 g of $CaCI_2$ and 100 g of $thiamin{\cdot}HCI$ in 1000 ml of distilled water.

Ganoderma lucidum이 생산하는 polygalacturonase의 유용 활용 방안을 위한 최적 생산조건을 검토한 결과는 다음과 같다. Polygalacturonase 생산을 위한 최적 배양조건은 $30^{\circ}C$, pH5.5에서 14일이었다. Polygalacturonase 생산을 위한 최적 영양조건은 pectin 10 g, soluble starch 10 g, yeast extract 1 g, peptone 2 g, phenylalanine 1 g, $KH_2PO_4$ 2 g, $MgSO_4$ 0.2 g, $CaCI_2$ 50 mg, $thiamin{\cdot}HCI\;100{\mu}g$, D.W. 1000ml이었다.

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