Changes in Hydrogen Donating Activities of the Extract from Hololeion maximowiczii Root by Drying Methods

건조방법에 따른 께묵뿌리 추출물의 수소공여능 조사

  • 최동연 (한양대학교 식품영양학과) ;
  • 도재호 (한국인삼연초연구원) ;
  • 이광승 (한국인삼연초연구원) ;
  • 양차범 (한양대학교 식품영양학과)
  • Published : 1994.04.01

Abstract

Hydrogen donating activities(HDA) of solvent extracts of Hololeion maximowiczii roots which were treated with freeze drying, hot air drying and hot air drying after steam blanching were investigated by the method of ${\alpha},{\alpha}'$-diphenyl-${\beta}$-picrylhydrazyl(DPPH). Hydrogen donating activity of hot air drying after steam blanching group showed the highest activity among three drying methods. Among the extracts of freeze dried root extracted with various solvents, color intensity at 420 nm of aqueous extract was highest, but hydrogen donating activity was shown in order of 50%, ethyl alcohol, methyl alcohol, water extracts. By heating the powder of freeze dried root at $100^{\circ}C$, color intensity of its water extract was decreased until 20 min, thereafter it was increased slowly but hydrogen donating activity was rapidly increased until 30 min. Hydrogen donating activity at the concentration of 6.05 mg/ml of 50% alcohol extract was similar to that of ${\alpha}-tocopherol$ at the concentration of $2{\times}10^{-4}M$. Comparison of hydrogen donating activity of the inner and outer solution after dialysis of water extract showed that the outer solution had a stronger hydrogen donating activity than those of the inner one.

께묵(Hololeion maximowicizii)뿌리를 냉동건조, 열풍건조 및 증숙후 열풍건조의 3가지 방법으로 건조한 후 용매추출하여 DPPH 방법에 의한 수소공여능이 변화를 조사하였다. 증숙한 후 열풍건조구가 가장 큰 수소공여능을 나타냈으며, 여러가지 용매 추출물중에서 물추출물의 색도가 가장 높았으나 수소공여능은 50% ethanol 추출물에서 가장 높게 나타났다. 냉동건조한 께묵뿌리를 $100^{\circ}C$에서 120분까지 열처리 한 위에 흡광도를 조사한 결과는 가열 20분까지는 감소하다가 증가하였으나 수소공여능은 계속 증가하였다. 50% ethanol 추출물(6.05mg/ml)은 $2{\times}10^{-4}M$${\alpha}-tocopherol$과 비슷한 수소공여능을 보였으며, 투석외액과 내액의 수공여능을 비교한 결과 외액이 내액보다 강하였다.

Keywords