Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Carbon and Nitrogen Source on Somatic Embryogenesis in Suspension Culture of Ligusticum chuanxiang Hort.

천궁의 현탁배양에서 탄소원과 질소원이 체세포배 형성에 미치는 영향

  • Chae, Young-Am (Agronomy Department and Research Center for New Biomaterials in Agriculture of Seoul National University) ;
  • Park, Sang-Un (Agronomy Department and Research Center for New Biomaterials in Agriculture of Seoul National University)
  • 채영암 (서울대학교 농학과, 농업생물신소재연구센터) ;
  • 박상언 (서울대학교 농학과, 농업생물신소재연구센터)
  • Published : 1994.03.31
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Abstract

This study was carried out to select the appropriate medium(especially, carbon and nitrogen source ) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of chuanxiang Hort. Suitable medium for somatic embryo formation was MS medium. The half strength MS medium was effective for somatic embryo development. Sucrose was the most effective carbon source for somatic embryo formation, however, production of somatic embryos was reduced at higher concentration of sucrose. Effects of suger was the same as sucrose. Somatic embryo formation was higher as the decrease of $NH_{4}NO_3$, and optimum ratio of $KNO_3\;:\;NH_4NO_3$ was 825 : 238mg /1. Regenerated plant was obtained in MS basal medium and survival late of plantlet was 60-70% after transplanted directly to the vermiculite.

1. 2.4-D 0.5mg/1에서 배발생캘러스 생장과 발생빈도가 가장 양호하였다. 2. 배지 중에서 MS배지가 체세포배 형성과 생육에 좋았으며, 1X MS배지 보다 1/2X MS배지가 배형성에 더 효과적이었다. 3. 탄소원으로는 sucrose가 효과적이었다. 농도는 1%처리가 가장 양호하였으며 농도가 높아짐에 따라 체세포배 형성이 억제되었다. 4. Sucrose 대신 sugar 처리에서도 체세포배 형성이 앙호하였다. 5. 질소원으로는 $KNO_3\;825mg\;/\;l$ 와 $NH_{4}NO_3$ 238mg /l 처리가 배발생에 가장 효과적이었다.

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