Journal of Oral Medicine and Pain
- 제19권2호
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- Pages.205-219
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- 1994
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- 2288-9272(pISSN)
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- 2383-8493(eISSN)
타액반 피검물에서 개인식별을 위한 DNA의 유전자형 검사
Typing of DNA Extracted from Cigarette Butts for Individual Identification
- Kyong-Kyue Yoon (Dept. of Oral Medicine and Oral Diagnosis, College of Dentistry, Yonsei University) ;
- Juck-Joon Hwang (Dept. of Oral Medicine and Oral Diagnosis, College of Dentistry, Yonsei University) ;
- Chong-Youl Kim (Dept. of Oral Medicine and Oral Diagnosis, College of Dentistry, Yonsei University)
- 발행 : 1994.06.01
초록
Cigarette butts from 5 smokers were gathered and then, placed in room temperature for 1, 3, 5, 7, 15 days. The possible use of the cigarette butts for individual identification was evaluated in sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 gene from the extracted DNA. 1. DNA extraction was possible in cigarette butts weree left in room temperature for 15days, so it can be applicatable to individual identification by polymerase chain reaction(PCR). 2. Amplification of X-Y homologous amelogenin gene by PCR made it possible to identify the sex in saliva stains (cigarette butts). 3. Amplification of D1S80 locus can be acquired from adding the boving serum albumin and hot start PCR procedures from forensic samples such as saliva stains (cigarette butts), so the AMP-FLPs examining is possible. 4. Genotype could be determined simply and rapidly using Amplitype
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